BIDMC Laboratory Manual -

Beth Israel Deaconess Medical Center
Laboratory Manual

Back to Main Page of Lab Manual

BONE MARROW PROCESSING
HEMATOLOGY LABORATORY
EAST CAMPUS

Bone marrow specimens are accepted and unstained aspirate smears made 7 days per week from 7:00 AM – 11:30 PM. Specimens after hours require medical director approval and arrangement.

Monday –Friday
Saturday
Sunday/Holidays
After Hours

Aspirate Staining
(Wright Giemsa) 7:00 AM – 3:30 PM
Staining not performed except with medical director approval

Iron Staining 7:00 AM – 3:00 PM
Batched, staining done once per day.
Staining not performed

Special Stains 7:00 AM – 2:00 PM
Medical Director approval required

No stains performed

Core Biopsy Delivered to Hematology Lab
(Hematology will deliver to Pathology) 7:00 AM –3:30 PM

Mon – Thurs after 3:00 PM tech will leave specimen at room temp at the Bone Marrow bench.

Friday after 3:00 PM tech will place in designated Cytology refrigerator (see Saturday schedule). Saturday 7:00 AM – 12 Noon

After 12 Noon Saturday, and on Sundays and Holidays, place specimen in designated Cytology refrigerator on the bottom left shelf. The designated refrigerator is adjacent to the Heme blood storage refrigerator in FN 305 STAT Lab. "CYTOLOGY SPECIMENS" is posted on the door.

Chromosomes
Cytogenetics Lab 8:30 AM –5:30 PM
After 5:30 PM leave specimen at room temp at the Bone Marrow bench for up to 16 hours. If 16 hour limit is to be exceeded follow Sunday/Holiday policy.
Saturday 7:30 AM – 5:00 PM

After 5:00 PM on Saturday and on Sundays and Holidays, specimens may be left at room temp at the Bone Marrow bench for up to 16 hours. If 16 hour time limit is to be exceeded on weekends or holidays, and/or for all new acute leukemia patients, page the Brigham and Women’s Cytogenetics Fellow On Call at:

617-732-5700 page number 13125.

This page is monitored 24 hours per day, 7 days per week.

Monday –Friday
Saturday
Sunday/Holidays
After Hours

Flow Cytometry Processed

8:30 AM – 2:00 PM
After 2:00 PM hold for 24 hrs at room temp at Bone Marrow bench. If 24 hrs is to be exceeded follow Sunday/Holiday policy.
Medical Director approval required Not accepted.

Emergencies require Medical Director approval. Upon approval tech will either arrange for in-house processing or send to Brigham and Women’s Hospital (see Sunday schedule). Hold for 24 hours at room temp at the Bone Marrow bench. Evenings, nights, Saturdays and Holidays require Medical Director approval. Upon approval, the lead tech will arrange for special in-house processing or send to Brigham and Women’s Hospital 617-732-5846. Send out procedure is posted at Flow Processing bench

last reviewed/revised: 3/20/2006

Back to Main Page of Lab Manual

	Monday –Friday	Saturday	Sunday/Holidays	After Hours
Aspirate Staining (Wright Giemsa)	7:00 AM – 3:30 PM	Staining not performed except with medical director approval
Iron Staining	7:00 AM – 3:00 PM Batched, staining done once per day.	Staining not performed
Special Stains	7:00 AM – 2:00 PM Medical Director approval required	No stains performed
Core Biopsy Delivered to Hematology Lab (Hematology will deliver to Pathology)	7:00 AM –3:30 PM Mon – Thurs after 3:00 PM tech will leave specimen at room temp at the Bone Marrow bench. Friday after 3:00 PM tech will place in designated Cytology refrigerator (see Saturday schedule).	Saturday 7:00 AM – 12 Noon After 12 Noon Saturday, and on Sundays and Holidays, place specimen in designated Cytology refrigerator on the bottom left shelf. The designated refrigerator is adjacent to the Heme blood storage refrigerator in FN 305 STAT Lab. "CYTOLOGY SPECIMENS" is posted on the door.
Chromosomes Cytogenetics Lab	8:30 AM –5:30 PM After 5:30 PM leave specimen at room temp at the Bone Marrow bench for up to 16 hours. If 16 hour limit is to be exceeded follow Sunday/Holiday policy.	Saturday 7:30 AM – 5:00 PM After 5:00 PM on Saturday and on Sundays and Holidays, specimens may be left at room temp at the Bone Marrow bench for up to 16 hours. If 16 hour time limit is to be exceeded on weekends or holidays, and/or for all new acute leukemia patients, page the Brigham and Women’s Cytogenetics Fellow On Call at: 617-732-5700 page number 13125. This page is monitored 24 hours per day, 7 days per week.